Iranian garden creates a complex system of spatial perception for its audiences by its structure. Organized layout of natural and man-made elements in Iranian garden space creation is one of the most essential components in creating special mental image in audiences of Iranian garden. The fundamental theorem of this study is based on surveying the impact of the structure of Mahan Shahzadeh Garden, as a sample of Iranian garden, possessing different spatial sequences on audiences perception due to motion in the garden space, which has been performed by identifying the garden spatial sequences, evaluating and criticizing the identified sequences based on assessment of audiences mental image. For this purpose, research method of text content analysis has been used for collecting theoretical data and case study method using questionnaire. Data were collected with a statistical population of 30 audiences of Mahan Shahzadeh Garden and statistical analysis was performed using SPSS software. Criticism in this study is of criterion oriented type in two quantity oriented and theory oriented sections are basis of critical analysis. The obtained results indicate that different sequences in this garden in respect of garden structure will create common mental images in audiences, since the percent of response of audiences to dynamic components resultant from the garden geometry, hierarchy of passing portal to main palace, creating pause in emerging audiences' emotional motion and so on has been higher than 50% and is indicative of their positive impact. At the end of the study, this result was obtained that Shahzadeh Mahan garden is a successful sample in creating pause feeling and other senses either in respect of physical or semantic motion.